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Image Search Results
Journal: Cancer Science
Article Title: Newcastle disease virus co‐expressing interleukin 7 and interleukin 15 modified tumor cells as a vaccine for cancer immunotherapy
doi: 10.1111/cas.13468
Figure Lengend Snippet: Construction of recombinant virus. A, Schematic representation of recombinant LX construct. The coding sequence interleukin 15 ( IL 15) and IL 7 was inserted into the sequence between P and M genes of LX genome. B, Multistep growth curve for LX and LX / IL (15+7). The virus titers were determined in triplicate by TCID 50 in DF ‐1 cells at 0, 8, 16, 24, 32 and 40 h post‐infection. The data shown are representative of the 3 experiments
Article Snippet: The expression s of IL7 and IL15 in the supernatants were measured using ELISA as described by
Techniques: Recombinant, Virus, Construct, Sequencing, Infection
Journal: Cancer Science
Article Title: Newcastle disease virus co‐expressing interleukin 7 and interleukin 15 modified tumor cells as a vaccine for cancer immunotherapy
doi: 10.1111/cas.13468
Figure Lengend Snippet: Expression of the interleukin 15 ( IL 15) and IL 7 in tumor cells infected with LX / IL (15+7). A, Irradiated B16 were incubated with LX / IL (15+7) or LX / RFP (100 HAU virus per 10 6 cells). After incubation for 24, 48, 72 and 96 h, the supernatants were collected and analyzed for the production of IL 15 and IL 7 (A) or IL 15‐2A‐ IL 7 (B) by ELISA
Article Snippet: The expression s of IL7 and IL15 in the supernatants were measured using ELISA as described by
Techniques: Expressing, Infection, Irradiation, Incubation, Virus, Enzyme-linked Immunosorbent Assay